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mcd99 When anti-mCD99 levels are hh, tumor cells are injected subcutaneously into the left flank of the mice (red arrow). Tumors are allowed to grow 3-4 weeks before the mice. Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40

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